Device

Part:BBa_K540000:Design

Designed by: Melanie Geffroy, Philippe Thomas   Group: iGEM11_LYON-Biosciences_INSA_ENS   (2011-09-15)

rcn-csgBAEFG, over-induces adherence in response to cobalt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 47
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2108
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Several internal iGEM restriction sites were initially present in the genes : EcoR1 in csgEFG, Pst1 in csgBA and rcn. They were eliminated in such a way that it does not affect the expression of the genes.


Source

Direct synthesis from Genecust, using the sequence of the genes as found in E.coli MC4100 genome.

References

1. Hammar, M., A. Arnqvist, Z. Bian, A. Olsen, and S. Normark. 1995. Expression of two csg operons is required for production of fibronectin- and congo red-binding curli polymers in Escherichia coli K-12. Mol. Microbiol. 18:661-670.

2. Robinson LS, Ashman EM, Hultgren SJ, Chapman MR. Secretion of curli fibre subunits is mediated by the outer membrane-localized CsgG protein Mol Microbiol. 2006 Feb;59(3):870-81.